Mechanism of axial stagger in myosin assembly.

Molecular Mechanism of Myosin Assembly

The Effect o f Diameter Ratio on the Performance of a Low, Stagger Axial-Compressor Stage

• Summary.-Low-speed tests have been carried out on a family of three single-stage compressors of different diameter ratios. The simple blade design tested showed considerable variations of performance as the diameter ratio was decreased from 0.65 to 0.35 but most of these variations are predictable by simple means and it is expected that they may be partially avoided by appropriate design meth...

Myosin V stepping mechanism.

We observe the myosin V stepping mechanism by traveling wave tracking. This technique, associated with optical tweezers, allows one to follow a scattering particle in a two-dimensional plane, with nanometer accuracy and a temporal resolution in the microsecond range. We have observed that, at the millisecond time scale, the myosin V combines longitudinal and vertical motions during the step. Be...

The mechanism of assembly of Acanthamoeba myosin-II minifilaments: minifilaments assemble by three successive dimerization steps

We used 90 degrees light scattering, analytical ultracentrifugation, and electron microscopy to deduce that Acanthamoeba myosin-II minifilaments, composed of eight molecules each, assemble by a novel mechanism consisting of three successive dimerization steps rather than by the addition of monomers or parallel dimers to a nucleus. Above 200 mM KCl, Acanthamoeba myosin-II is monomeric. At low io...

The Mechanism of Assembly ofAcanthamoeba Myosin-II Minifilaments: Minifilaments Assemble by Three Successive Dimedzation Steps

We used 90 ° light scattering, analytical ultracentrifugation, and electron microscopy to deduce that Acanthamoeba myosin-II minifilaments, composed of eight molecules each, assemble by a novel mechanism consisting of three successive dimerization steps rather than by the addition of monomers or parallel dimers to a nucleus. Above 200 mM KCI, Acanthamoeba myosin-II is monomeric. At low ionic st...